Search results for "a DNA"
showing 4 items of 4 documents
RNase H1 and H2 are differentially regulated to eliminate RNA-DNA hybrids
2019
SUMMARYRNA-DNA hybrids are tightly regulated to ensure genome integrity. The RNase H enzymes, RNase H1 and H2, contribute to chromosomal stability through the removal of RNA-DNA hybrids. Loss of RNase H2 function is implicated in human diseases of the nervous system and cancer. To better understand RNA-DNA hybrid dynamics, we have focused on elucidating the regulation of the RNase H enzymes themselves. Using yeast as a model system, we demonstrate that RNase H1 and H2 are controlled in different manners. RNase H2 is regulated in a strict cell cycle dependent manner, both in terms of its R-loop removal, and ribonucleotide excision repair functions. RNase H1, however, can function independent…
Direct measurement of cell-free DNA from serially collected capillary plasma during incremental exercise
2014
To investigate the kinetics of cell-free DNA (cfDNA) due to exercise, we established a direct real-time PCR for the quantification of cfDNA from unpurified capillary plasma by amplification of a 90- and a 222-bp multilocus L1PA2 sequence. Twenty-six male athletes performed an incremental treadmill test. For cfDNA measurement, capillary samples were collected serially from the fingertip preexercise, during, and several times postexercise. Venous blood was drawn before and immediately after exercise to compare capillary and venous cfDNA values. To elucidate the strongest association of cfDNA accumulations with either cardiorespiratory or metabolic function during exercise, capillary cfDNA val…
Genetic structure and differentiation from early bronze age in the mediterranean island of sicily: Insights from ancient mitochondrial genomes
2022
Sicily is one of the main islands of the Mediterranean Sea, and it is characterized by a variety of archaeological records, material culture and traditions, reflecting the history of migrations and populations’ interaction since its first colonization, during the Paleolithic. These deep and complex demographic and cultural dynamics should have affected the genomic landscape of Sicily at different levels; however, the relative impact of these migrations on the genomic structure and differentiation within the island remains largely unknown. The available Sicilian modern genetic data gave a picture of the current genetic structure, but the paucity of ancient data did not allow so far to make p…
Rendimiento neuropsicológico en pacientes que demandan tratamiento por consumo de drogas
2015
El estudio sobre los déficits neuropsicológicos en adicciones ha cobrado una especial relevancia en las últimas décadas, lo que se debe fundamentalmente a sus implicaciones en aspectos tan importantes como el pronóstico terapéutico y el diseño de estrategias adecuadas de intervención (Landa, Fernández y Tirapu, 2004; Madoz, Ochoa y Martínez, 2009). Sin embargo, y a pesar de que el policonsumo de sustancias constituye un patrón de consumo cada vez más prevalente en la población general (DGPNSD, 2013), tanto los servicios asistenciales de demanda de tratamiento como la investigación previa, suelen informar de la sustancia principal por la que el paciente acude a tratamiento, no ofreciendo inf…